Extraction and characterization of pepsin-soluble collagen from different mantis shrimp species

The objective of this study was to investigate the yield and characteristics collagen protein extracted from muscle four different species mantis shrimp: Miyakella nepa, Harpiosquilla harpax, Erugosquilla woodmasoni, Odontodactylus cultrifer. Mantis shrimp by using a pepsin-solubilization technique, with 0.5 M acetic acid 5% pepsin enzyme. highest M. nepa (0.478 ± 0.06%), which significantly greater (p < 0.05) than that H. O. cultrifer, E. woodmasoni (0.313 0.03%, 0.123 0.02%, 0.015 0.00%, respectively). freeze-dried appeared as thin fibers, formed an opaque film. pepsin-soluble (PSC) analyzed gel electrophoresis. results showed all contained type I collagen, consisting β, α1, α2 subunits average molecular weights 250, 145, 118 kDa, respectively. solubility that, for NaCl, had relative in 2% NaCl (80.20 4.95%). In contrast, decreased at higher concentrations. However, terms pH, pH 3 (91.32 5.14%), its pH. FT-IR spectroscopy used compare model compound. Five wavenumbers spectrum were identified: Amide A (3,406–3,421 cm−1), amide B (2,916–2,940 (1,639–1,640 II (1,539–1,570 III (1,234–1,250 cm−1).